Competent Cells
MilliporeSigma™ Novagen™ RosettaBlue™ (DE3) Competent Cells
RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ BL21(DE3) Competent Cells
Achieve greater efficiency, reproducibility and convenience with Novagen prepared competent cells. BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high-level protein expression using a T7 RNA polymerase-IPTG induction system.
MilliporeSigma™ Novagen™ Rosetta-gami™ B Competent Cell Set
Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance the expression of eukaryotic proteins. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells
BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
MilliporeSigma™ Novagen™ Rosetta™ 2 Competent Cell Set
Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ Tuner™ (DE3) pLacI Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture. Contains the pLacI plasmid producing extra Lac repressor.
MilliporeSigma™ Novagen™ HMS174 (DE3) pLysS Competent Cells
HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. T7 lysozyme expression suppresses basal T7 expression.
MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) Competent Cells
Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Tuner™ DE3 Competent Cells
Tuner host strains are lacZY deletion mutants of BL21, which enable adjustable levels of protein expression throughout all cells in a culture.
MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.
MilliporeSigma™ Novagen™ Veggie BL21 (DE3) Singles™ Competent Cells
Protease-deficient hosts (BL21) for protein expression; Manufactured free of animal-derived media and components
Invitrogen™ TOP10F' Electrocomp™ Kit
TOP10F' Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F' episome.
Invitrogen™ MC1061/P3 Chemically Competent E. coli
MC1061/P3 Chemically Competent E. coli are used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.1, or any other supF-containing vector).
MilliporeSigma™ Novagen™ DE3 Competent Cell Set
For Protein Expressions
Invitrogen™ MultiShot™ StripWell Mach1™ T1 Phage-Resistant Chemically Competent E. coli
MultiShot StripWell Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations.